Coding

Part:BBa_K4335003

Designed by: Yuning Luo   Group: iGEM22_UESTC-BioTech   (2022-09-30)
Revision as of 12:29, 10 October 2022 by Hang Zhang (Talk | contribs)


ChlamyHgR

We used the Rbcs2 promoter and terminator, and since the Rbcs2 Promotor Rhizobium-derived transcription factor was low, we added the UTR of the heat shock promoter HSP70A Promotor to enhance the promoter and thus the heterologous expression of the Staygold fluorescent protein StayGold, a green fluorescent protein (GFP) from the jellyfish family Endocystis, was chosen as our reporter fluorescent protein because it is orders of magnitude more photostable than any currently available fluorescent protein, and because the excitation and emission wavelengths of Staygold do not overlap with the chloroplast fluorescence of Chlamydomonas reinhardtii itself. 

  <figure>
       <img src="T--UESTC-China--result-egl7-3.png" width="30%" style="float:center">
       <figcaption>


       </figcaption>
   </figure>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 782
    Illegal NgoMIV site found at 890
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None