Part:BBa_K4158010

Psrtf1-GFP

This part contains RBS, GFPuv coding site and a promoter regulated by SRTF1 and Progesterone((1S,3aS,3bS,9aR,9bS,11aS)-1-Acetyl-9a,11a-dimethyl-1,2,3,3a,3b,4,5,8,9,9a,9b,10,11,11a-tetradecahydro-7H-cyclopenta[a]phenanthren-7-one) and works as the reporter plasmid to confirm exist of Progesterone and SRTF1.

RBS+GFPuv sequence is the same as BBa_K4158011.

We designed Psrtf-GFP(BBa_K4158010), and confirmed its activity in vitro. This part is the reporter plasmid used for progesterone detection. It encodes GFP gene in the downstream of the binding site of SRTF1, the transcriptional factor specific to progesterone.

Promoter and SRTF1 binding site: Sequence information was cited from paper[1].Consequently, the promoter sequence was the same as BBa_J32102 and the SRTF1 binding site was the same as No part name specified with partinfo tag.) was successfully achieved</b>. we achieved a project based on BioBricks, which is an important standard component in synthetic biology.</li> </ul>

Thus, we succeeded in engineering SRTF1 regulated gfp reporter plasmid(BBa_K4158010). (For more details, go to https://2022.igem.wiki/waseda-tokyo/results#progesterone-detection.)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 13
  Illegal NheI site found at 36
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 826
  Illegal XhoI site found at 529
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Sankar K et al. A progesterone biosensor derived from microbial screening. ACS Sens. 7(4):1132-1137(2022).