Coding

Part:BBa_K4461002

Designed by: SHIH-HSUN, YANG   Group: iGEM22_NYCU-Taipei   (2022-10-08)
Revision as of 11:57, 10 October 2022 by YU-TANG-CHANG (Talk | contribs)


nirBDC promoter

The expression of the nirB promoter is low at lag and early log for aerobic condition and fis inhibition. After the mid-log phase, it is activated by the anaerobic condition. However, in the late log and stationary phase, the expression would be inhibited by IHF. Note that if not using LB but a minimal medium would result in rather a low expression.

This is the nirBDC mRNA expression-Time graph.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None