Coding

Part:BBa_K4229013

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-28)
Revision as of 10:55, 10 October 2022 by NikitaEdel (Talk | contribs)


TnaA with N-terminal SnoopCatcher


TnaA codes for a tryptophanase. This tryptophanase turns L-tryptophan into either Indol. This biobrick consists of a version of TnaA, which is fused with the snoopcatcher(BBa_K4229009) on the N-terminal of the protein. With that, we made it able to be recruted into the wiffelball we used in our project.

Grafik indigo weier bg.png
CatchedProteins.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 977
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 977
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 977
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 977
    Illegal AgeI site found at 142
    Illegal AgeI site found at 228
    Illegal AgeI site found at 1600
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None