Regulatory

Part:BBa_K4175007

Designed by: Li Xianxiu, Zhang Wanying   Group: iGEM22_ZJUintl-China   (2022-08-27)
Revision as of 10:30, 10 October 2022 by JasLee24 (Talk | contribs)


MND promoter

In our project, this promoter is used in IL-6_scfv-Notch-Gal4KRAB (BBa_K4175008), IL-6R-Notch-Gal4KRAB (BBa_K4175010) and IL-6R-PD-1 (BBa_K4175012) expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 60
    Illegal PstI site found at 55
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 60
    Illegal BamHI site found at 76
    Illegal XhoI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None