Regulatory

Part:BBa_K4241791

Designed by: Vicera Christian Nelson Tejedor   Group: iGEM22_HKU_HongKong   (2022-10-10)
Revision as of 09:27, 10 October 2022 by Registry (Talk | contribs)

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Nitrogen Starvation Inducible promoter 2 (Microalgae)

This part is CvNDI2, a nitrogen starvation induced promoter from Chlorella vulgaris to be used in microalgal expression chassis. This part is derived from the following publication: https://www.nature.com/articles/s41598-020-69620-9

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 630
    Illegal PstI site found at 679
    Illegal PstI site found at 749
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 87
    Illegal PstI site found at 630
    Illegal PstI site found at 679
    Illegal PstI site found at 749
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 238
    Illegal XhoI site found at 663
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 630
    Illegal PstI site found at 679
    Illegal PstI site found at 749
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 630
    Illegal PstI site found at 679
    Illegal PstI site found at 749
    Illegal NgoMIV site found at 902
    Illegal AgeI site found at 832
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None