Part:BBa_K4169006

BBa_K4169006

HepT: a RNase like toxin

HepT is a toxin protein that can be effectively inhibited the growth of bacteria.The HepT toxin could function as an RNase with a RX4-6H active motif and cleave mRNA to inhibit cell growth. Complete with HepT (HZAU-China 2021:BBa_K3733010) was used in this study.

Usage and Biology

The RNA thermometer has a special sequence capable of forming stem-loop structures at 27 ° C and below and opening at 37 ° C. At the same time, there is a specific binding site for RNase E on the sequence that can form the secondary structure. At the transcriptional level, if this sequence is present on mRNA, it is able to bind RNase E and thus degrade the entire RNA strand. Therefore, we introduce the thermometer into the suicide module to achieve temperature sensitive suicide.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 103
  Illegal PstI site found at 127
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 103
  Illegal PstI site found at 127
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 103
  Illegal PstI site found at 127
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 103
  Illegal PstI site found at 127
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

Hoynes-O'Connor A, Hinman K, Kirchner L, Moon TS. De novo design of heat-repressible RNA thermosensors in E. coli. Nucleic Acids Res. 2015 Jul 13;43(12):6166-79.