Coding

Part:BBa_K4137010

Designed by: YA-JHIH KO   Group: iGEM22_GEMS_Taiwan   (2022-10-07)
Revision as of 01:55, 8 October 2022 by Registry (Talk | contribs)

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CcdA coding sequence + Myc Tag

The above sequence encodes for the CcdA protein, a antitoxin formany bacteria including Escherichia coli. By forming CcdA-CcdB complex by binding with CcdB, CcdB loses its ability to interact with DNA gyrase thus losing its toxicity. The Myc tag is a polypeptide protein tag added for protein purification and identification, which serves similar functions as the 6xHis tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None