![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4137010
Designed by: YA-JHIH KO Group: iGEM22_GEMS_Taiwan (2022-10-07)
CcdA coding sequence + Myc Tag
The above sequence encodes for the CcdA protein, a antitoxin formany bacteria including Escherichia coli. By forming CcdA-CcdB complex by binding with CcdB, CcdB loses its ability to interact with DNA gyrase thus losing its toxicity. The Myc tag is a polypeptide protein tag added for protein purification and identification, which serves similar functions as the 6xHis tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |