Generator
Part:BBa_K4325015
Designed by: Laiyi Teng Group: iGEM22_SZPT-CHINA (2022-09-30)
J23102-RBS003422-gshF-T0
Description
The composite part is a generator consisting of a J23102 promoter and gshF.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 223
Illegal EcoRI site found at 805
Illegal EcoRI site found at 1240
Illegal EcoRI site found at 1738
Illegal PstI site found at 64
Illegal PstI site found at 94
Illegal PstI site found at 451 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 223
Illegal EcoRI site found at 805
Illegal EcoRI site found at 1240
Illegal EcoRI site found at 1738
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 64
Illegal PstI site found at 94
Illegal PstI site found at 451
Illegal NotI site found at 1883
Illegal NotI site found at 2083 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 223
Illegal EcoRI site found at 805
Illegal EcoRI site found at 1240
Illegal EcoRI site found at 1738
Illegal XhoI site found at 2064 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 223
Illegal EcoRI site found at 805
Illegal EcoRI site found at 1240
Illegal EcoRI site found at 1738
Illegal PstI site found at 64
Illegal PstI site found at 94
Illegal PstI site found at 451 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 223
Illegal EcoRI site found at 805
Illegal EcoRI site found at 1240
Illegal EcoRI site found at 1738
Illegal PstI site found at 64
Illegal PstI site found at 94
Illegal PstI site found at 451
Illegal NgoMIV site found at 1665
Illegal NgoMIV site found at 2296 - 1000COMPATIBLE WITH RFC[1000]
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 223
Illegal EcoRI site found at 805
Illegal EcoRI site found at 1240
Illegal EcoRI site found at 1738
Illegal PstI site found at 64
Illegal PstI site found at 94
Illegal PstI site found at 451 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 223
Illegal EcoRI site found at 805
Illegal EcoRI site found at 1240
Illegal EcoRI site found at 1738
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 64
Illegal PstI site found at 94
Illegal PstI site found at 451
Illegal NotI site found at 1883
Illegal NotI site found at 2083 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 223
Illegal EcoRI site found at 805
Illegal EcoRI site found at 1240
Illegal EcoRI site found at 1738
Illegal XhoI site found at 2064 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 223
Illegal EcoRI site found at 805
Illegal EcoRI site found at 1240
Illegal EcoRI site found at 1738
Illegal PstI site found at 64
Illegal PstI site found at 94
Illegal PstI site found at 451 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 223
Illegal EcoRI site found at 805
Illegal EcoRI site found at 1240
Illegal EcoRI site found at 1738
Illegal PstI site found at 64
Illegal PstI site found at 94
Illegal PstI site found at 451
Illegal NgoMIV site found at 1665
Illegal NgoMIV site found at 2296 - 1000COMPATIBLE WITH RFC[1000]
2022 SZPT-China
Biology
This composite part is a generator consisting of a J23102 (BBa_J23102) promoter and gshF (BBa_K4325003).
Usage
The J23102 promoter and gshF were connected and inserted into the pSEVA331 expression vector so that gshF expressed the novel bifunctional enzyme GshF, which directly catalyze the synthesis of glutathione by the three amino acids of Cys, Glu and Gly.
Characterization
1.Verification of gshF in E. coli
[edit]
Categories
Parameters
None |