Coding
fiatluxA

Part:BBa_K4239003:Design

Designed by: Guillaume FULCONIS   Group: iGEM22_INSA_Lyon1   (2022-10-06)
Revision as of 19:53, 6 October 2022 by Gfulconis (Talk | contribs) (→‎Design Notes)


Enhanced luciferase substrate forming units fiatluxA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 504
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1023


Design Notes

The Igem restriction site EcoRI is removed from iluxA.

Some mutations were realize in luxA K22E, T119A, S178A


Fiatlux genes come from ilux genes (C, D, A, B, E). They were modified to remove every Igem restriction site (EcoR1, Xba1, Spe1 and Pst1) included in genes. They were also adapted to include the biobrick format.

Source

azaze

References

Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.