Coding
fiatluxD

Part:BBa_K4239002:Design

Designed by: Guillaume FULCONIS   Group: iGEM22_INSA_Lyon1   (2022-10-05)
Revision as of 17:02, 6 October 2022 by Gfulconis (Talk | contribs) (→‎References)


Enhanced luciferase subunits fiatluxD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Every Igem restriction sites (EcoR1, Xba1, Spe1 and Pst1) were removed from the part. Codon optimization is done for E.coli and D.solani.



Source

fiatlux is made up with fiatluxA, fiatluxB and fiatluxE

References

Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.