Part:BBa_K1724002
MerR
The mercury –sensing regulatory protein, MerR(wild type), which regulates mercury resistance operons in Gram-negative bacteria, is subjected to directed evolution in an effect to generate a MerR mutant that responds to Cadmium ion but not mercury.That is, the MerR mutant is the cadmium-sensing regulatory protein. To get MerR mutant, Oligonucleotide-directed mutagenesis is used to introduce random mutations into the key metal-binding regions of MerR. Finally, Getting the generated Cd-specific MerR mutants appears to be unique.
The quantification data of MerR/CadA operon can be seen in BBa_K1724000.[[1]]
IMPROVEMENT: iGEM Peshawar 2017 has gotten this part synthesized and has submitted it to the registry for future use by teams.
Bioinformatic characterization – iGEM Edinburgh-UHAS_Ghana 2022
We characterized the structure and metal interaction of this part. We compared the metal binding properties of this mutated form of MerR (henceforth referred to as mut_MerR) to wild-type MerR, to see if the affinity for the heavy metal ion remains constant. We also generated a novel structure for mut_MerR, as well as a structure bound to pmerT, to show it can theoretically function in vivo.
test
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 258
Illegal AgeI site found at 360 - 1000COMPATIBLE WITH RFC[1000]
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