Coding

Part:BBa_K200001

Designed by: Royah Vaezi, Dineka Khurmi   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 14:20, 29 September 2009 by Droche (Talk | contribs) (→‎Requirements)

Dam methylase -> Dam

The methylase encoded by the dam gene ([http://en.wikipedia.org/wiki/Dam_(methylase) Dam methylase]) transfers a methyl group from S-adenosylmethionine to the N6 position of the adenine residues in the sequence GATC. When methylation occurs in the recognition site of a particular group of restriction endonuclease including MboI, this protects the DNA from cleavage.

Usage and Biology

DAM methylase (DNA adenine methylase) adds a methyl group to the adenine of the sequence 5'-GATC-3'in newly synthesized DNA. Dam methylases are important for several biological functions:

  • [http://en.wikipedia.org/wiki/DNA_replication DNA replication]
  • [http://en.wikipedia.org/wiki/Mismatch_repair DNA mismatch repair]
  • Regulation of protein expression.

In the E.ncapsulator project, Dam methylases are used to protect the DNA from being destroyed by restriction enzymes DpnII and TaqI.

Figure 1: Action of Dam methylase (source: #Dam1)
Figure: Action of Dam methylase (source: #Dam1)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

<biblio>#Dam1 http://fangman-brewer.genetics.washington.edu/hemimethylation.html</biblio>


[edit]
Categories
Parameters
n/aDam methylase -> Dam