Translational_Unit

Part:BBa_K4241023

Designed by: Ng Tsz Chun   Group: iGEM22_HKU_HongKong   (2022-09-30)
Revision as of 15:28, 30 September 2022 by Registry (Talk | contribs)

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6xHis_SUMO_RFP

This is the expression of a T7 tagged SUMO-RFP fusion, which the RFP could be his-tag pulled down and purified via Ulp1 cleavage and second round of His-tag pulldown.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 155
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1050
    Illegal AgeI site found at 1162
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None