Composite
Part:BBa_K4343092
Designed by: huanyu li Group: iGEM22_NNU-China (2022-10-09)
pUC-HUH-Scp2-EhElo9(PFABin)
EhElo9 encodes ∆-9 elongase from Emiliania huxleyi which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1431
Illegal XhoI site found at 3465
Illegal XhoI site found at 3498
Illegal XhoI site found at 8261 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3653
Illegal NgoMIV site found at 7321
Illegal AgeI site found at 889
Illegal AgeI site found at 1543
Illegal AgeI site found at 1642
Illegal AgeI site found at 2003
Illegal AgeI site found at 4706
Illegal AgeI site found at 5067
Illegal AgeI site found at 6644 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 1546
Illegal BsaI site found at 2183
Illegal BsaI site found at 5247
Illegal BsaI site found at 5784
Illegal BsaI site found at 6647
Illegal BsaI site found at 7470
Illegal BsaI.rc site found at 401
Illegal BsaI.rc site found at 1540
Illegal BsaI.rc site found at 6641
Illegal BsaI.rc site found at 7464
Illegal BsaI.rc site found at 7734
Illegal BsaI.rc site found at 8896
Illegal BsaI.rc site found at 9280
Illegal SapI.rc site found at 1908
Illegal SapI.rc site found at 4972
[edit]
Categories
Parameters
None |