![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4192113
Designed by: Lehan Dong Group: iGEM22_CAU_China (2022-09-29)
Plac-RBS-cdg
This part is used to test the role of cdg (BBa_K4192010) in biofilm production. We used IPTG to induce its expression, and then used crystal violet to dye 96 well plates. The results are shown in the figure below.
The effect of this part is not significant,which means it is hard to work alone. However, it shows obvious enhancement effect when it interacts with gacA gene (BBa_K4192011).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 468
Illegal BamHI site found at 459
Illegal XhoI site found at 557
Illegal XhoI site found at 1001 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 392
Illegal AgeI site found at 959 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 410
Illegal BsaI.rc site found at 977
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Categories
Parameters
None |