Coding

Part:BBa_K4214003

Designed by: Jol Tekoniemi   Group: iGEM22_Stockholm   (2022-10-08)
Revision as of 18:58, 8 October 2022 by Registry (Talk | contribs)

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hGIF with LacI promotor and 6xHis-tag

LacI (BBa_J04500) and Human GIF with His6-tag. This part is a composite part from BBa_K4214002 with the addition of LacI sequence(BBa_J04500). The LacI sequence is used in order to induce our protein expression with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 485
    Illegal BsaI.rc site found at 1165
    Illegal SapI site found at 1155


[edit]
Categories
Parameters
None