Composite
Part:BBa_K4343085
Designed by: Xiner Liu Group: iGEM22_NNU-China (2022-10-09)
pUC-HUH-Scp2-PsElo9
PsElo9 gene encodes ∆-9 elongase from Pavlova salina. This enzyme can catalyze the elongation of C18:2 to C20:2 in Y. lipolytica.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1431
Illegal XhoI site found at 3465
Illegal XhoI site found at 3498
Illegal XhoI site found at 7865 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3653
Illegal AgeI site found at 889
Illegal AgeI site found at 1543
Illegal AgeI site found at 1642
Illegal AgeI site found at 2003
Illegal AgeI site found at 4706
Illegal AgeI site found at 5067
Illegal AgeI site found at 6209 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 1546
Illegal BsaI site found at 2183
Illegal BsaI site found at 5247
Illegal BsaI site found at 5784
Illegal BsaI site found at 6212
Illegal BsaI site found at 6353
Illegal BsaI.rc site found at 401
Illegal BsaI.rc site found at 1540
Illegal BsaI.rc site found at 6206
Illegal BsaI.rc site found at 7068
Illegal BsaI.rc site found at 7338
Illegal BsaI.rc site found at 8500
Illegal BsaI.rc site found at 8884
Illegal SapI.rc site found at 1908
Illegal SapI.rc site found at 4972
[edit]
Categories
Parameters
None |