Composite

Part:BBa_K4343077

Designed by: Yue Shu   Group: iGEM22_NNU-China   (2022-10-09)
Revision as of 12:08, 9 October 2022 by Registry (Talk | contribs)

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pUC-HUH-IntC-FmDes12

FmDes12 gene encodes a ∆-12 desaturase from Fusarium moniliform, which can catalyze C18:1 to C18:2. This gene is expressed in Y. lipolytica.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6773
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3344
    Illegal XhoI site found at 3377
    Illegal XhoI site found at 8239
    Illegal XhoI site found at 9155
    Illegal XhoI site found at 9446
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3532
    Illegal NgoMIV site found at 6797
    Illegal AgeI site found at 1422
    Illegal AgeI site found at 1521
    Illegal AgeI site found at 1882
    Illegal AgeI site found at 4585
    Illegal AgeI site found at 4946
    Illegal AgeI site found at 6088
    Illegal AgeI site found at 7544
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1425
    Illegal BsaI site found at 2062
    Illegal BsaI site found at 5126
    Illegal BsaI site found at 5663
    Illegal BsaI site found at 6091
    Illegal BsaI site found at 7547
    Illegal BsaI.rc site found at 1419
    Illegal BsaI.rc site found at 6085
    Illegal BsaI.rc site found at 7541
    Illegal BsaI.rc site found at 8041
    Illegal BsaI.rc site found at 8051
    Illegal BsaI.rc site found at 9469
    Illegal SapI site found at 8967
    Illegal SapI.rc site found at 1787
    Illegal SapI.rc site found at 4851


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