Composite
Part:BBa_K4343077
Designed by: Yue Shu Group: iGEM22_NNU-China (2022-10-09)
pUC-HUH-IntC-FmDes12
FmDes12 gene encodes a ∆-12 desaturase from Fusarium moniliform, which can catalyze C18:1 to C18:2. This gene is expressed in Y. lipolytica.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 6773
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 3344
Illegal XhoI site found at 3377
Illegal XhoI site found at 8239
Illegal XhoI site found at 9155
Illegal XhoI site found at 9446 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3532
Illegal NgoMIV site found at 6797
Illegal AgeI site found at 1422
Illegal AgeI site found at 1521
Illegal AgeI site found at 1882
Illegal AgeI site found at 4585
Illegal AgeI site found at 4946
Illegal AgeI site found at 6088
Illegal AgeI site found at 7544 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 1425
Illegal BsaI site found at 2062
Illegal BsaI site found at 5126
Illegal BsaI site found at 5663
Illegal BsaI site found at 6091
Illegal BsaI site found at 7547
Illegal BsaI.rc site found at 1419
Illegal BsaI.rc site found at 6085
Illegal BsaI.rc site found at 7541
Illegal BsaI.rc site found at 8041
Illegal BsaI.rc site found at 8051
Illegal BsaI.rc site found at 9469
Illegal SapI site found at 8967
Illegal SapI.rc site found at 1787
Illegal SapI.rc site found at 4851
[edit]
Categories
Parameters
None |