Part:BBa_K4343084

pUC-HUH-Scp2-PpElo9

PpElo9 gene encodes ∆-9 elongase from Pavlova pinguis. This enzyme can catalyze the elongation of C18:2 to C20:2 in Y. lipolytica.

Characterisation

We integrated PpElo9 controlled by the TEF promoter to the SCP2 site of the genome of Y. lipolytica through this segment. Then we detected the proportion of C18:2 and C20:2 by gas chromatography (GC) and test the activity of ∆-9 elongase according to the results.

Result

C18:2 produced by them decreased from 62.7% to 34%, and C20:2 produced by them reached 32%.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 6489
  Illegal XhoI site found at 1431
  Illegal XhoI site found at 3465
  Illegal XhoI site found at 3498
  Illegal XhoI site found at 7844
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3653
  Illegal NgoMIV site found at 7021
  Illegal AgeI site found at 889
  Illegal AgeI site found at 1543
  Illegal AgeI site found at 1642
  Illegal AgeI site found at 2003
  Illegal AgeI site found at 4706
  Illegal AgeI site found at 5067
  Illegal AgeI site found at 6209
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1
  Illegal BsaI site found at 1546
  Illegal BsaI site found at 2183
  Illegal BsaI site found at 5247
  Illegal BsaI site found at 5784
  Illegal BsaI site found at 6212
  Illegal BsaI site found at 6796
  Illegal BsaI.rc site found at 401
  Illegal BsaI.rc site found at 1540
  Illegal BsaI.rc site found at 6206
  Illegal BsaI.rc site found at 7047
  Illegal BsaI.rc site found at 7317
  Illegal BsaI.rc site found at 8479
  Illegal BsaI.rc site found at 8863
  Illegal SapI.rc site found at 1908
  Illegal SapI.rc site found at 4972