Coding

Part:BBa_K4241013

Designed by: Ng Tsz Chun   Group: iGEM22_HKU_HongKong   (2022-09-28)
Revision as of 16:08, 28 September 2022 by Registry (Talk | contribs)

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6xHis-thrombin-T7Tag

This contains adds the 6xHis-Thrombin-T7 tag, however it does not contains a start codon and must be used in frame with a prmoter with a Met initiation site. The thrombin can be used to cleave away the 6xHis tag. Moreover, T7 tag was proven to increase the yield of recombinant protein production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 55
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None