RBS

Part:BBa_K4241012

Designed by: Ng Tsz Chun   Group: iGEM22_HKU_HongKong   (2022-09-24)
Revision as of 14:50, 24 September 2022 by Kngtszchun (Talk | contribs)


TIR-2 translation initiation region 2

https://www.nature.com/articles/s42003-020-0939-8 This is a enhanced RBS/start codon region which was proven to yield higher protein expression in bacterial chassis.

This will initiate the protein translation at the start codon including a Met residue. Like any other pET plasmid expression chassis, it would have this scar.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None