Coding
fuGFP-link

Part:BBa_K4488011

Designed by: Donovan Wu, Jackie Yau, Oliver Nicholls, Jasmin Li   Group: iGEM22_Sydney_Australia   (2022-09-30)
Revision as of 09:14, 9 October 2022 by Jali2097 (Talk | contribs)


Fusion of free-use GFP with CBDclos (cellulose-binding domain) at the C-terminal end with a linker

The construct can be cloned into an expression vector such as pET28c in E.coli to produce a fusion protein of fuGFP with CBDclos. The fuGFP sequence is towards the N terminus of the protein with CBDclos (BBa_K1321341) downstream followed by a stop codon. Recognition sites for BamHI and BsaI are present before the RBS allowing golden gate cloning. XhoI and BsaI are also present downstream of the stop codon.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 163
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None