Composite
pLac+RBS+C
Part:BBa_K4137013
Designed by: YA-JHIH KO Group: iGEM22_GEMS_Taiwan (2022-10-07)
ccdB toxin secretion pathway
This part consists of a pLac promoter, RBS, a ccdB-6xHis coding complex, and a terminator (BBa_B1006). This part produces a constant expression of ccdB toxin under the LB medium, inhibiting the GyrA subunit of DNA gyrase and prevents negative supercoiling of DNA. An accumulation of ccdB without sufficient suppression by antitoxin ccdA leads to risks in host viability.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 289
[edit]
Categories
Parameters
None |