Composite
pLac+RBS+C

Part:BBa_K4137013

Designed by: YA-JHIH KO   Group: iGEM22_GEMS_Taiwan   (2022-10-07)
Revision as of 02:34, 8 October 2022 by Registry (Talk | contribs)

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ccdB toxin secretion pathway

This part consists of a pLac promoter, RBS, a ccdB-6xHis coding complex, and a terminator (BBa_B1006). This part produces a constant expression of ccdB toxin under the LB medium, inhibiting the GyrA subunit of DNA gyrase and prevents negative supercoiling of DNA. An accumulation of ccdB without sufficient suppression by antitoxin ccdA leads to risks in host viability.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 289


[edit]
Categories
Parameters
None