Reporter
Part:BBa_K182101
Designed by: Zuzana Hruskova and Calum de Burgh Group: iGEM09_Aberdeen_Scotland (2009-07-07)
pLux-Lac Hybrid with GFP Reporter and Double Terminator
Hybrid Promoter with LacO site and pLux-Lac sites for Quorum Sensing reporting via GFP.
As it also responds to LacI, this part acts as an AND logic gate, relying upon the inputs of AHL and IPTG (or some other form of LacI repression) in order to produce the GFP output
Comprised of parts BBa_I751502 (as developed by Tokyo Tech) upstream and BBa_E0840 downstream.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 747
[edit]
Categories
Parameters
None |