Plasmid_Backbone
pSB4C5

Part:pSB4C5:Experience

Designed by: Reshma Shetty   Group: iGEM06_Bangalore   (2007-02-26)
Revision as of 09:42, 20 September 2009 by Katriona87 (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of pSB4C5

User Reviews

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Reshma Shetty

pSB4C5 is functional. When pSB4C5-I52002 is transformed into TOP10 cells, no colonies are obtained as expected. When pSB4C5-I52002 is cut with NotI, self-ligated (to eliminate ccdB positive selection marker) and transformed, lots of colonies are obtained. Moreover, pSB4C5-I52002 has been used to successfully assemble BioBrick parts. When miniprepped pSB4C5-I52002 gives high miniprep yield similar to a high copy plasmids.

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Aberdeen_Scotland 2009

Mixed digestion results; digestion with EcoRI and PstI worked well in some cases and complete digestion was observed, however, in other cases incomplete digestion was observed. When pSB4C5-I52002 is transformed into XL1-blue cells, colonies are obtained. This is an unexpected results since this part contains the ccdB 'death' gene.

UNIQ66cf548158464330-partinfo-00000003-QINU