Composite
Part:BBa_K4268020:Design
Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski Group: iGEM22_SUNY_Oneonta (2022-09-30)
S-TIP 37 Ghost Phagemid with Biotin-tagged Capsid
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3810
Illegal PstI site found at 7523 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 6161
Illegal NheI site found at 10697
Illegal PstI site found at 3810
Illegal PstI site found at 7523 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5174
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3810
Illegal PstI site found at 7523 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3810
Illegal PstI site found at 7523
Illegal NgoMIV site found at 3450
Illegal NgoMIV site found at 3589
Illegal NgoMIV site found at 4545
Illegal NgoMIV site found at 12194
Illegal AgeI site found at 14225 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Golden Gate Assembly Level 3
Source
The coding sequences were derived from the S-TIP37 genome.