Composite

Part:BBa_K4137008

Designed by: YA-JHIH KO   Group: iGEM22_GEMS_Taiwan   (2022-08-20)
Revision as of 03:11, 8 October 2022 by Stuberosum ryan (Talk | contribs)


mleR expressing construct

This construct contains a T7 promoter, a RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This construct produce and purifies mleR, the regulator of CcdA, which produces when malate acid is presented.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 49
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None