Composite

Part:BBa_K4268020

Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski   Group: iGEM22_SUNY_Oneonta   (2022-09-30)
Revision as of 00:38, 1 October 2022 by Registry (Talk | contribs)

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S-TIP 37 Ghost Phagemid with Biotin-tagged Capsid

This is the complete cyanophage phagemid with multi-transcription units of Coding Sequence Expression Cassette 2 and Coding Sequence Expression Cassette 3. This phagemid has been optimized to have a biotin tag for biotin-strepavidin interaction applications.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3810
    Illegal PstI site found at 7523
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6161
    Illegal NheI site found at 10697
    Illegal PstI site found at 3810
    Illegal PstI site found at 7523
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5174
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3810
    Illegal PstI site found at 7523
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3810
    Illegal PstI site found at 7523
    Illegal NgoMIV site found at 3450
    Illegal NgoMIV site found at 3589
    Illegal NgoMIV site found at 4545
    Illegal NgoMIV site found at 12194
    Illegal AgeI site found at 14225
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None