Composite

Part:BBa_K4268019:Design

Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski   Group: iGEM22_SUNY_Oneonta   (2022-09-30)
Revision as of 00:28, 1 October 2022 by Registry (Talk | contribs)

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S-TIP 37 Ghost Phagemid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3810
    Illegal PstI site found at 7463
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6101
    Illegal NheI site found at 10637
    Illegal PstI site found at 3810
    Illegal PstI site found at 7463
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5114
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3810
    Illegal PstI site found at 7463
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3810
    Illegal PstI site found at 7463
    Illegal NgoMIV site found at 3450
    Illegal NgoMIV site found at 3589
    Illegal NgoMIV site found at 4545
    Illegal NgoMIV site found at 12134
    Illegal AgeI site found at 14165
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Golden Gate Assembly Level 3



Source

The coding sequences were derived from the S-TIP37 genome.


References