Composite
Part:BBa_K4268018
Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski Group: iGEM22_SUNY_Oneonta (2022-09-30)
Revision as of 00:25, 1 October 2022 by Jazmine124 (Talk | contribs)
Biotin Tagged Capsid and Core Multi-Transcriptional Unit
Usage and Biology
This is a Golden Gate Assembly Level 2 part. This part joins transcriptional units 6, 7, and 8 – tail tubular proetin B, internal core protein, and the tail fiber-lile protein respectively.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3806
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3806
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5170
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3806
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3806
Illegal NgoMIV site found at 3446
Illegal NgoMIV site found at 3585
Illegal NgoMIV site found at 4541 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |