Plasmid
pCG004

Part:BBa_K4321005

Designed by: Kulay Janneh   Group: iGEM22_Guelph   (2022-09-28)
Revision as of 18:42, 28 September 2022 by Registry (Talk | contribs)

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E.coli to Bacillus subtilis shuttle plasmid (pCG004)

Shuttle plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9048
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 3615
    Illegal EcoRI site found at 6835
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3615
    Illegal EcoRI site found at 6835
    Illegal EcoRI site found at 9048
    Illegal NheI site found at 3451
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 9054
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3615
    Illegal EcoRI site found at 6835
    Illegal EcoRI site found at 9048
    Illegal BglII site found at 361
    Illegal BglII site found at 6306
    Illegal XhoI site found at 365
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9048
    Illegal suffix found in sequence at 2
    Illegal EcoRI site found at 3615
    Illegal EcoRI site found at 6835
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9048
    Illegal EcoRI site found at 3615
    Illegal EcoRI site found at 6835
    Illegal XbaI site found at 9063
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6234
    Illegal BsaI.rc site found at 5170
    Illegal SapI.rc site found at 2338
    Illegal SapI.rc site found at 7459


[edit]
Categories
//chassis/prokaryote/bsubtilis
//plasmid/expression
//plasmidbackbone/copynumber/high
//plasmidbackbone/expression/inducible
Parameters
None