Coding

Part:BBa_K4197021

Designed by: [laure] [lamothe]   Group: iGEM22_Toulouse_INSA-UPS   (2022-10-07)
Revision as of 07:27, 7 October 2022 by Registry (Talk | contribs)

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Ana o 3 expression at the surface of E. coli cells sortable by FACS using mSCARLET-I

OmpA_Ana o 3 fusion + mSCARLET-I with ihfb800 promoter

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1520
    Illegal EcoRI site found at 1734
    Illegal XbaI site found at 1505
    Illegal XbaI site found at 1651
    Illegal PstI site found at 213
    Illegal PstI site found at 224
    Illegal PstI site found at 342
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1520
    Illegal EcoRI site found at 1734
    Illegal NheI site found at 1696
    Illegal PstI site found at 213
    Illegal PstI site found at 224
    Illegal PstI site found at 342
    Illegal NotI site found at 1512
    Illegal NotI site found at 2690
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1520
    Illegal EcoRI site found at 1734
    Illegal BglII site found at 1585
    Illegal BamHI site found at 1728
    Illegal XhoI site found at 2699
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1520
    Illegal EcoRI site found at 1734
    Illegal XbaI site found at 1505
    Illegal XbaI site found at 1651
    Illegal PstI site found at 213
    Illegal PstI site found at 224
    Illegal PstI site found at 342
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1520
    Illegal EcoRI site found at 1734
    Illegal XbaI site found at 1505
    Illegal XbaI site found at 1651
    Illegal PstI site found at 213
    Illegal PstI site found at 224
    Illegal PstI site found at 342
    Illegal AgeI site found at 329
    Illegal AgeI site found at 1475
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2361


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Parameters
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