Composite

Part:BBa_K4345024

Designed by: Luka Van den Berghe   Group: iGEM22_KU_Leuven   (2022-10-08)
Revision as of 18:03, 8 October 2022 by Registry (Talk | contribs)

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5' UTR of prfA with ccdA fused to sfGFP

Design of a thermoregulated translation of antitoxin ccdA. Fluorescent protein sfGFP was linked to the antitoxin with a rigid linker for visualisation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1229
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 649
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
//cds/reporter/gfp
//regulation/constitutive
//rna/riboswitch
Parameters
biology
control
emission
excitation