Part:BBa_K4387003

Negative Control of Nitric Oxide Sensing Genetic Circuit

Usage and Biology

This composite part consists of a superfolder GFP, the transcriptional regulator NorR, and a double forward terminator. We chose a high-copy backbone from Twist Bioscience for this part. To assess the proper induction of our construct by nitric oxide, we removed the pNorV promoter to create a negative control. With the removal of the promoter, the presence of nitric oxide should not affect the expression of GFP. Therefore this part was used for the autofluorescence of the EcN cultures in the following constructs:

• Nitric Oxide Sensing Genetic Circuit With the ETH promoter pNorV
• Nitric Oxide Sensing Genetic Circuit With One Ribosomal Binding Site
• Nitric Oxide Sensing Genetic Circuit With Two Ribosomal Binding Sites
• Nitric Oxide Sensing Genetic Circuit With Three Ribosomal Binding Sites

This construct was tested in the bacterial strain E.coli Nissle 1917.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 439
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]