Part:BBa_K4268003:Experience

The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.

Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Capsid Protein with Biotin Tag insert. The insert length is 780bp and the predicted size of the PCR product when using VF/VR primers is 1085 bp.

The gel indicates that colonies 1 and 2 are likely to contain the correct insert and thus, the Capsid Protein with Biotin Tag was successfully cloned into a Level 0 Golden Gate Assembly basic part.

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