Composite
Part:BBa_K238018
Designed by: Annelien Verfaillie Group: iGEM09_KULeuven (2009-09-22)
EF ligation with promotor and terminator
this is another intermediate of part K238006. in this part a promoter was put before part K238017.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2006
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 89
Illegal NgoMIV site found at 443
Illegal NgoMIV site found at 1166
Illegal AgeI site found at 594
Illegal AgeI site found at 1131
Illegal AgeI site found at 1592
Illegal AgeI site found at 2034 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |