Composite

Part:BBa_K4345020

Designed by: Elias Maris   Group: iGEM22_KU_Leuven   (2022-09-30)
Revision as of 16:21, 30 September 2022 by Registry (Talk | contribs)

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5' UTR of hsp17 with ccdA fused to sfGFP

Design of the thermoregulated translation of antitoxin ccdA. Fluorescent protein sfGFP was linked to the antitoxin for visualisation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1149
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 569
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
//cds/reporter/gfp
//regulation/constitutive
//rna/riboswitch
Parameters
biology
control
emission
excitation