Composite

Part:BBa_K4122025

Designed by: Aiqi Luo   Group: iGEM22_IvyMaker-China   (2022-09-28)
Revision as of 13:01, 28 September 2022 by Registry (Talk | contribs)

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SP-PETase-His-SC-T-ADH2

+ SP-MHETase-cMyc-SNC-T-PGK1

+ SP-CBM-V5 -ST-ST-SNT-ST-7813

Compose of 3 Parts：

P-SP-PETase-His-Spycatcher-T-ADH2

P-SP-MHETase-cMyc-Snoopcatcher-T-PGK1

SP-CBM-V5-ST-ST-SNT-ST-7813

In this assembly, SP is signal peptide, the same as last year (BBa_K4122014). Different from BBa_K4122020, we exchanged the position of tags and catchers.Besides, we also moved V5 tag before the spytag to fold correctly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None