Composite

Part:BBa_K4345004:Design

Designed by: Luka Van den Berghe   Group: iGEM22_KU_Leuven   (2022-09-27)
Revision as of 14:42, 29 September 2022 by LVdB (Talk | contribs) (→‎References)


NarX mutant fused to mPapaya with a rigid linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2153
    Illegal PstI site found at 659
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2153
    Illegal PstI site found at 659
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2153
    Illegal XhoI site found at 260
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2153
    Illegal PstI site found at 659
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2153
    Illegal PstI site found at 659
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

NarX mutant was designed based on literature (Cavicchioli et al., 1995). mPapaya was obtained from a collegues plasmid.

References

Cavicchioli, R., Schro¨der, I., Schro¨der, S., Constanti, M., & Gunsalus, R. P. (1995). The NarX and NarQ Sensor-Transmitter Proteins of Escherichia coli Each Require Two Conserved Histidines for Nitrate-Dependent Signal Transduction to NarL. JOURNAL OF BACTERIOLOGY, 177(9), 2416–2424.

Cheung, J., & Hendrickson, W. A. (2009). Structural Analysis of Ligand Stimulation of the Histidine Kinase NarX. Structure, 17(2), 190–201. https://doi.org/10.1016/J.STR.2008.12.013