Composite

Part:BBa_K4165233

Designed by: Omnia AlaaEldeen Abdelazeem   Group: iGEM22_CU_Egypt   (2022-10-08)
Revision as of 09:21, 8 October 2022 by Omnia Alaa11 (Talk | contribs)


His - UBE2N

This biobrick consists of T7 promotor (BBa_K3633015), RBS (BBa_K4165016), His 6-His tag (BBa_K4165020), UBE2N (BBa_K4165011), T7 Terminator (BBa_K731721), The His tag was attached to the UBE2N coding sequence to serve in the purification using NI-NTA column.

Usage and Biology

this part is a Ubiquitin-conjugating E2 ligase which has a role in the ubiquitination cascade for protein degradation. It has been used in this project to target and degrade both tau and Aβ proteins which are both considered the main causes of Alzheimer’s Disease pathogenesis.

Source

Synthesized

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None