Composite

Part:BBa_K4165233

Designed by: Omnia AlaaEldeen Abdelazeem   Group: iGEM22_CU_Egypt   (2022-10-08)
Revision as of 09:16, 8 October 2022 by Registry (Talk | contribs)

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His - UBE2N

This biobrick consists of T7 promotor (BBa_K3633015), RBS (BBa_K4165016), His 6-His tag (BBa_K4165020), UBE2N (BBa_K4165011), T7 Terminator (BBa_K731721), The His tag was attached to the UBE2N coding sequence to serve in the purification using NI-NTA column.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None