Device

Part:BBa_K4407010

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-Nanjing   (2022-10-05)
Revision as of 08:28, 8 October 2022 by Xuleyu (Talk | contribs)


Pthl-dps，Expression of dps with Pthl promoter

This part is responsible for expressing the dps protein under the control of a Pthl promotor, a ribosome binding site (RBS) and a terminator. It is composed of BBa_K3443002 (Pthl), BBa_K103015 (RBS), BBa_K4407001 (dps), and BBa_K3585002 (terminator). dps protects the bacteria from oxidative damage. It protects DNA against oxidative damage by binding with Fe2+ to prevent it from reacting with H2O2 (Fenton’s reaction). In our project, we used this device to enhance the viability of C. tyrobutyricum in aerobic environment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None