Coding
CcdA

Part:BBa_K4137000:Design

Designed by: Clifford S Chang   Group: iGEM22_GEMS_Taiwan   (2022-08-20)
Revision as of 06:34, 5 October 2022 by Stuberosum ryan (Talk | contribs) (→‎Design Notes)


CcdA-6xHis Antitoxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The 6xHis purification tag was added to the C-terminal of the protein. The sequence has been codon optimized for E. coli. An alternative version, BBa_K4137010, is the CcdA sequence with the Myc tag added to the N-terminal of the protein.

Source

https://www.ncbi.nlm.nih.gov/nuccore/U51588.1

References

https://www.ncbi.nlm.nih.gov/nuccore/U51588.1