Composite

Part:BBa_K4399010:Design

Designed by: Duoqing LIN   Group: iGEM22_Worldshaper-Shanghai   (2022-09-27)
Revision as of 02:56, 28 September 2022 by Registry (Talk | contribs)

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PLexA35S-SbDEL-Tnos


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1266
    Illegal PstI site found at 17
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1266
    Illegal PstI site found at 17
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1266
    Illegal BglII site found at 678
    Illegal BamHI site found at 921
    Illegal BamHI site found at 990
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1266
    Illegal PstI site found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1266
    Illegal PstI site found at 17
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Induciable transcription of SbDEL (BBa_K4399004) is initiated by PLexA35S (BBa_K3900024) and terminated by Tnos (BBa_P10401).


Source

The sequence is based on BBa_K3900024, BBa_K4399004 and BBa_P10401.

References