Coding

Part:BBa_K1054002

Designed by: Jiasheng Wang   Group: iGEM13_ZJU-China   (2013-09-10)
Revision as of 20:36, 27 September 2013 by Stargazin (Talk | contribs)

bacteriophage PBSX holin

Holin is an essential protein for host lysis by bacteriophage. This part is cloned from Bacillus subtilis subsp. subtilis str. 168. Its activity can by blocked by another protein called lrgA (BBa_K1054024) and lrgB (BBa_K1054003). We can use this feature to prevent horizontal gene transfer of certain plasmid.

Holin 2013zjuchina.png

Growth curve of BL21 E.coli cells induced by different concentrations of IPTG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/celldeath
Parameters
n/abacteriophage PBSX holin