Regulatory
blr promot

Part:BBa_K238000

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-07-14)
Revision as of 16:33, 27 July 2009 by AnneV (Talk | contribs)

YcgF/E regulated promoter region

ycgF/E regulated promoter region ycgf is a cytoplasmic receptor which responds to blue light. it contains a Blue light Using FAD-Domain (BLUF) and an EAL domain. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to ycgE repressor through its EAL-domain releasing the reporssor from the promoter region. see reference for more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes and development23: 522-534 (2009)

there will be an attempt to remove the SpeI site at position 178-183


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 178
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 178
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 178
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 178
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aYcgF/E regulated promoter region