Composite
Part:BBa_K4179020
Designed by: Baraah Rashed Group: iGEM22_Technion-Israel (2022-10-06)
Luc_Blast_OraCell
The composite part comprises a Blasticidin S deaminase(BBa_K1943015), which is a Blasticidin resistance gene with the Kozak sequence. Downstream to this under the CMV(BBa_I712004) which is a constitutive promoter, an SV40PolyA terminator(BBa_K4235020) is cloned. Together in this composite, a cTNT promoter(BBa_K4179021) is found, downstream to the 8XGTIIC motifs(BBa_K4179017) followed by the luciferase gene(BBa_I712019) and the low most part SV40 Poly(A)(BBa_K4235020). Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 213
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1090
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Categories
Parameters
//awards/composite_part/nominee
None |