Coding

Part:BBa_K4152001

Designed by: Qinting Lin   Group: iGEM22_TJUSLS_China   (2022-09-28)
Revision as of 03:12, 8 October 2022 by MirjaWang (Talk | contribs)


PK_MT1

To improve the performance of Proteinase K, we designed many Proteinase K mutant genes. PK_MT1 is a complicated mutation of Proteinase K, which contains 5 mutation sites: N257C-S173Y-T16C-T20D-V198F.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 235
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 235
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None