Coding
PcOSM

Part:BBa_K4147000

Designed by: Denisse Chacon Ramirez   Group: iGEM22_Tec-Chihuahua   (2022-09-30)
Revision as of 23:14, 7 October 2022 by Denisse (Talk | contribs)

PcOSM: An osmotin from Piper colubrinum (An antimicrobial peptide)

This osmotin (PcOSM) is a plant defense protein with a molecular weight of ~25 kDa. PcOSM belongs to pathogenesis related-5 (PR-5) family, which is accumulated in response to both biotic and abiotic stresses [1]. This osmotin has exhibited significant inhibitory activity against the oomycete pathogen Phytophthora capsici. This peptide is able to inhibit fungal growth through inhibition of hyphal growth, spore germination, spore lysis, and reduction in viability of spore [2].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 579
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 271
  • 1000
    COMPATIBLE WITH RFC[1000]



References

1. Geetha RG, Krishnankutty Nair Chandrika S, Saraswathy GG, Nair Sivakumari A, Sakuntala M. ROS Dependent Antifungal and Anticancer Modulations of Piper colubrinum Osmotin. Molecules. 2021; 26(8):2239. https://doi.org/10.3390/molecules26082239

2. Chowdhury, S., Basu, A. & Kundu, S. Cloning, Characterization, and Bacterial Over-Expression of an Osmotin-like Protein Gene from Solanum nigrum L. with Antifungal Activity Against Three Necrotrophic Fungi. Mol Biotechnol 57, 371–381 (2015). https://doi.org/10.1007/s12033-014-9831-4

3. Mani T, Sivakumar KC, Manjula S. Expression and functional analysis of two osmotin (PR5) isoforms with differential antifungal activity from Piper colubrinum: prediction of structure--function relationship by bioinformatics approach. Molecular biotechnology. 2012;52(3):251–261.

4. Narasimhan ML, Coca MA, Jin J, κ.ά. Osmotin is a homolog of mammalian adiponectin and controls apoptosis in yeast through a homolog of mammalian adiponectin receptor. Molecular cell. 2005;17(2):171–180.

5. Ali, T.; Yoon, G.H.; Shah, S.A.; Lee, H.Y.; Kim, M.O. Osmotin attenuates amyloid beta-induced memory impairment, tau phosphor-ylation and neurodegeneration in the mouse hippocampus. Sci. Rep. 2015, 5, 1–7.

6. Watanabe, T. Therapeutic properties of the new phytochemical osmotin for preventing atherosclerosis. Vessel Plus 2020, 4.

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